Kits for isolation of viral RNA for PCR or manual extraction
Save time by isolating viral nucleic acids from tracheal swabs or other starting materials with the deltaPREP Anipath DNA/RNA Kit for automated extraction on the KingFisher FLEX platform or for manual extraction with the deltaPREP Virus TS RNA Kit. With the the new deltaPREP product line we offer viral extraction kits for nucleic acid extraction and purification. Based on a new type of chemistry, it ranges from manual to automated solutions for nucleic acid extractions of different kinds of starting materials. The extraction procedure of the automated kit takes place on a magnetic particle processor and allows the parallel extraction of DNA /RNA for up to 96 samples.
The manual kit deltaPREP TS RNA Kit is designed for the fast isolation of viral RNA from tracheal swabs.
Both kits allow the efficient and fast extraction of viral RNA based on an optimized and patented extraction chemistry.
NOTE: For evaluation and research use only. Not for medical, diagnostics and use on humans. For more information contact IST AG
Benefits & Characteristics
- Patented DC Technology: rapid lysis and efficient binding
- Efficient and sensitive recovery of viral NA
- Extraction method based on the use of Spin Filters or magnetic particle
- Optimum removal of inhibitors ensures trouble-free use of nucleic acids in subsequent applications
- Fast extraction protocols (51 minutes/for 96 samples based on the KingFisher Flex magnetic particle processor or approx. 30 minutes for manual extraction)
The deltaPREP Virus TS RNA Kit is designed for fast isolation of viral RNA from tracheal swabs. The extraction procedure is based on a new and patented chemistry. The procedure combines lysis of starting material with subsequent binding of viral nucleic acids onto the surface of a Spin Filter membrane. After several washing steps the viral nucleic acids are eluted from the membrane by using RNase free water. Extraction chemistry and extraction protocol are optimized to get a maximum yield. The kit also contains a Carrier RNA.
The detection limit for certain viruses depends on individual procedures, for example in house PCR or commercially used detection assays. We highly recommend the usage of own internal control DNA or RNA (IC DNA/RNA) or own internal standards (low copy) respectively, as well as positive and negative controls to monitor the purification, amplification, and detection processes.
Kit Components: Lysis Solution CBV, Washing Solution LS, Proteinase K, Carrier RNA, Spin Filter, Receiver Tubes, RNase-free water
Successfully tested for: Corona Virus (Covid-19), HIV, HBV, Influenza
The deltaPREP AniPath DNA/RNA Kit KFFLX is designed for automated isolation of viral or bacterial DNA and RNA from different kinds of starting material on the KingFisher FLEX, a magnetic particle processor for NA extraction of up to 96 samples per run. The extraction procedure is based on a new patented chemistry. The procedure combines lysis of starting material with subsequent binding of DNA and RNA on surface modified magnetic particles. After washing steps, the Nucleic Acid is eluted from the magnetic particles by using water.
The extraction process runs in two steps: The first step includes automated sample lysis, followed by the second step which performs automated nucleic acid extraction. The extraction procedure takes place on the magnetic particle processor KingFisher FLEX and allows the parallel extraction of up to 96 samples.
MAG Suspension F, Lysis Solution V, Binding Solution V, Carrier Mix, Washing Solution HS & L, RNase-free water
Successfully tested for:
Corona Virus (Covid-19), Canine Distemper Virus, Bluetongue-Virus, Bonine Viral Diarrhoea Virus, Porcine Circovirus 2, Koi Herpes Virus, Porcine Reproductive and Respiratory Syndrome Virus, Staphylococcus aureus, E. coli, Chlamydia spp., Leptospira interrogans, Lawsonia intracellularis, Mycobacterium avium paratuberculosis, Toxoplasma gondii
The detection limit for certain viruses depends on the individual procedures, e.g. in-house PCR or commercially used detection assays.