IST Innuscreen

We supply ready-to-use kits for molecular diagnostics and for your preferred nucleic acid extraction technology. 

Sophisticated tool-optimized specifically for isolating genomic DNA from tiny samples and from highly contaminated forensic specimens. Extraction has been successfully tested for a multitude of starting materials, such as blood, traces of blood, hair, hair roots, beard stubble, finger nails, postage stamps, cigarette butts, chewing gum, traces of sperm, swab samples and finger prints on a variety of surfaces. 

Using the innuPREP Bacteria DNA Kit-IPC16 allows researchers to isolate bacterial DNA both from bacterial cultures (gram+ and gram-) and from tissue samples. The corresponding extraction routine takes place on the InnuPure C16 touch automation system. Following external lysis, the sample is transferred to pre-filled, sealed Reagent Strips/Plates. The subsequent processes of binding the nucleic acid to magnetic particles, washing and final elution are fully automated. The option of initially lysing the cell walls with lysozyme, especially if working with gram+ bacteria, is recommended. Yields and purity of the isolated bacterial DNA are excellent.

The processing of diverse starting materials with only one universal extraction kit offers enormous flexibility and is of special interest in veterinary diagnostics. The innuPREP AniPath DNA/RNA Kit – KFFLX is optimized for the automated extraction of pathogen DNA and RNA using the KingFisher FLEX system (Thermo Fisher Scientific) and can handle most kinds of starting material: cell-free body fluids such as serum and plasma, whole blood, nasopharyngeal swabs, tissue and fecal samples. Up to 96 samples can be purified simultanously within 60 min. Thanks to the innovative extraction chemistry developed and patented by IST Innuscreen, nucleic acid binding to magnetic particles happens in presence of low salt concentrations and the extraction process results in best yields of pure DNA/RNA.

Nucleic acids finally eluted into the 96-well plate are ready to use for following downstream application such as real-time PCR. The included Carrier Mix composed of Carrier RNA, Internal Control DNA (IC DNA) and RNA (IC RNA) serves as extraction control.

Using the innuPREP Plant DNA Kit-FX, it is possible to isolate highly pure genomic DNA from various plant materials. After efficient homogenization using SpeedMill PLUS or another homogenizer, or using a mortar and liquid nitrogen, the plant material is lysed and both proteins and polysaccharides are effectively removed in a precipitation step. The lysate is filtered and then transferred to the plates, which are already self-filled or filled using CyBio FeliX. Using magnetic particle separation, nucleic acid extraction is automated on the CyBio FeliX. The final eluate is highly pure, free of magnetic particles and can therefore be used directly in subsequent applications, e.g. qPCR. The kit has already been successfully tested on leaves and herbs, corn, cornmeal and seeds.